Proteomics，2013

Genetic changes in muscle protein following hybridization between Haliotis diversicolor reeve Japan and Taiwan populations revealed using a proteomic approach

Guilan Di,Weiwei You^_，Jinjin Yu, Dexiang Wang ^_，Caihuan Ke

简介：

采用双向电泳和质谱技术，对杂色鲍台湾群体（TT）、日本群体（RR）和杂交子代“东优1号（TR）”足部肌肉蛋白进行了研究，经过PDQuest 8.0 数据分析，日本群体平均为924±7个点，台湾群体平均为861±11个点，杂交群体平均为882±9个点。聚类分析表明：RR和TR先聚为一类，然后再和TT聚为一类。共鉴别出46个蛋白点，其中有15个点来自鲍的数据库，识别率为33.6%。鉴别出的蛋白质主要参与肌肉的收缩和调节、能量代谢、胁迫应答等。参与能量代谢的蛋白包括ATP合成酶β亚基、果糖-1，6-二磷酸醛缩酶、磷酸丙糖异构酶、烯醇酶、精氨酸激酶等。鉴别出参与能量代谢的有差异表达的蛋白，杂交后代表现出相加性和超显性。鉴别出2个应激蛋白，即热激蛋白Hsp70和Cu/Zn-超氧化酶歧化酶。热激蛋白Hsp70在三个鲍家族足部肌肉中的表达量是TR>RR>TT，杂交后代呈现超显性。Cu/Zn-超氧化酶歧化酶在三个鲍家族足部肌肉中的表达量是RR>TR>TT，杂交后代呈现相加性。

Abstract：

Protein expression patterns were compared in a Japan and Taiwan population of Haliotis diversicolor and in a hybrid between them using 2DE and MALDI-TOF-TOF analyses. Using the software PDQuest, 924 ± 7 protein spots were detected in the Japan population (RR), 861 ± 11 in the Taiwan population (TT), and 882 ± 9 in the F1 hybrid (TR). RR and TR were clustered together, but the distance between RR and TT was the maximum using hierarchical cluster analysis. A total of 46 gel spots were identified and a total of 15 spots matched with abalone proteins (a 33.6% identification rate). Hybrid exhibiting additivity or overdominance accounted for 73.9% of these 46 identified proteins. The 46 differentially expressed proteins were shown to be involved in major biological processes, including muscle contraction and regulation, energy metabolism, and stress response. The proteins involved in energy metabolism included adenosine triphosphate (ATP) synthase β subunit, fructose 1, 6-bisphosphate aldolase, triosephosphate isomerase, enolase, arginine kinase, and tauropine dehydrogenase. These proteins exhibited additivity in their offspring. The proteins involved in stress responses included HSP Hsp70 (exhibiting overdominance in the offspring) and Cu/Zn-superoxide dismutase (exhibiting additivity). These results suggested that proteomic approach is suitable for analysis of heterosis and functional prediction of abalone hybridization.