Fish& Shellfish Immunology，2013
Phagocytosis and respiratoryburst activity of haemocytes from the ivory snail, Babylonia areolata
Guilan Di, Zhaoxia Zhang , Caihuan Ke
简介：
研究了方斑东风螺（Babylonia areolata）血细胞对副溶性血弧菌(Vibrio parahaemol-yticus)和酵母(Saccaromces cerevisia)的吞噬和呼吸爆发等。实验结果表明，方斑东风螺血细胞对副溶性血弧菌的吞噬能力强于酵母菌，颗粒细胞对副溶性血弧菌的吞噬率（PP）均略高于对酵母的吞噬率，并且对副溶性血弧菌的吞噬指数（PI）明显高于对酵母的吞噬指数。透明细胞也是如此。对于同一种菌，颗粒细胞的吞噬率都明显高于透明细胞的吞噬率，而吞噬指数却没有明显差异。借助流式细胞仪技术，吞噬作用和呼吸爆发能力随着时间的延长而增加，90分钟后吞噬作用不再增加，呼吸爆发能力120分钟后不再增加。
Abstract：
Haemocytes from the ivory snail, Babylonia areolata phagocytized Saccharomyces cerevisiae and Vibrio parahaemolyticus after 30 min. Haemocytes phagocytized V. parahaemolyticus at a greater rate than they phagocytized S. cerevisiae. The phagocytic rate (PP) of V. parahaemolyticus by granulocytes to was a little higher than that of S. cerevisiae. The phagocytic index (PI) of V. parahaemolyticus by granulocytes was significantly higher than that of S. cerevisiae. The same was true of hyalinocytes. The PP of granulocytes was significantly higher than that of hyalinocytes for each pathogen. No difference in PI was observed in granulocytes and hyalinocytes. Two defense mechanisms of B. areolata were quantified using flow cytometry. Haemocyte phagocytosis was quantified using fluorescent microbeads and respiratory burst activity was measured using H2O2 increases detected by 2′, 7′-dichlorofluorescein diacetate. Both phagocytosis and respiratory burst activity of the haemocytes increased over time. After 90 min the phagocytic rate no longer increased. In the case of respiratory burst, the greatest increase in fluorescence occurred between 30 and 120 min, no further increase was seen after 120 min. These results showed unequivocally that a native (unstimulated) haemocyte oxidative burst was active in B. areolata. The aim of this study was to further the knowledge of immunology in gastropods.