新疆两色金鸡菊提取物体外清除自由基活性研究

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摘要:

以乙醇为溶剂,采用超声波辅助提取新疆两色金鸡菊.提取物经不同极性溶剂进行萃取,获得不同极性溶剂提取物.同时考察这些提取物在体外清除DPPH、ABTS+、羟自由基(*OH)和超氧自由基(*O2-)的能力.结果显示,新疆两色金鸡菊氯仿提取物、乙酸乙酯提取物和正丁醇提取物清除DPPH的能力(SC50)依次是(7.6×10~3±4.02)μg/mL、(1.75×10~2±3.64)μg/mL、(1.34×10~2±2.75)μg/mL和(2.28×10~2±4.64)μg/mL;清除ABTS+自由基的能力(SC50)依次是(4.53×10~3±6.37)μg/mL、(1.26×10~2±2.97)μg/mL、(90.72±3.16)μg/mL和(1.28×10~2±5.78)μg/mL;清除*OH自由基的能力(SC50)依次是(3.86×10~3±5.79)μg/mL、(14.49±0.576)μg/mL、(13.80±0.672)μg/mL和(22.41±1.13)μg/mL.在不同自由基清除体系中,与阳性对照维生素C和叔丁基对羟基茴香醚相比,新疆两色金鸡菊不同极性溶剂提取物表现出强弱不同的自由基清除能力.

The Coreopsis tinctoria flowering tops (CTF) in Xinjiang was extracted with ethanol by ultrasonic-assisted extraction. The crude alcoholic extract was concentrated and suspended in distilled water, and defatted with petroleum ether. The aqueous layer was successively extracted with chloroform, ethyl acetate, and n-butanol. Forethmore, the radicals scaven- ging activities of the CTF extracts were investigated through DPPH, ABTS, ~ OH and " 02- assays. The results showed that the chloroform extract(CE), ethyl acetate extract (EAE) and n-butanol extract (BE) for DPPH radical scavenging activities (SC^0) of 7.6X103~ 4.0Z,l. 75X102-k- 3.64,1.34X10Zzk 2. 75, 2. 28 X lOZ :i: 4.64 ttg/mL, respectively. TheCE; EAE and BE for ABTS+ radical scavenging activities (SC^0) of 4.53 X 103 ~ 6.37,1.26 X 10z ~ 2.97,90.72 ~ 3.16, 1.28 X 10~ ~ 5.78 t^g/mL, respectively. The CE, EAE and BE for " OH radical scavenging activities (SC50) of 3.86X 103 ~ 5.79,14.49 + O, 576,(13.80 ~ 0, 672) and (22.41 5: 1, 13) ~tg/mL, respectively. The extracts of CTF showed different radical-scavenging capacity, which compared with vitamin C and butylated hydroxyanisole (BHA) in different radical scavenging system.

作者:

乔克宁 王露 冶晓童 冯玉洁 任盟乔 姚新成

机构地区:

石河子大学 药学院 石河子大学新疆特种植物药资源重点实验室

出处:

《Betway官方客服学报:自然科学版》 CAS 北大核心  2015年第6期129-133,共5页

基金:

新疆生产兵团重点领域科技攻关项目(2014BA031) 石河子大学高层次人才科研启动项目(RCZX201328) 石河子大学大学生研究训练计划(SRP201510759068)

关键词:

两色金鸡菊 溶剂提取物 自由基清除能力 自由基活性

Coreopsis tinctoria Nutt various solvent extracts free radical-scavenging activity

分类号:

R9 [医药卫生—药学] 


新疆两色金鸡菊提取物体外清除自由基活性研究.pdf

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