磁场条件下Fe2+对产聚唾液酸大肠杆菌的诱变选育
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摘要：
以产聚唾液酸(polysialic acid,PSA)的大肠杆菌(Escherichia coli)为出发菌株,在磁场条件下利用Fe2+对该菌种进行多轮诱变选育,获得具有高产性能的诱变菌株,在5L发酵罐进行发酵验证.结果表明:在0.5T磁场条件下,Fe2+的最佳诱变质量浓度为90mg·L-1;经过5轮诱变和选育,PSA的摇瓶发酵水平从1.09g·L-1提高到2.24g·L-1;5L发酵罐发酵水平从4.79g·L-1提高到9.22g·L-1,产量提高了92%.
Compound mutagenesis using magnetic field and Fe 2＋ was carried out several rounds to gain a high-yielding Escherichia coli strain of polysialic acid （PSA） fermentation and the high-yielding strain was verified in 5 L fermentor. The results showed that the optimal Fe 2＋ ion concentration was 90 mg·L -1 in the compound mutagenesis using magnetic field of 0.5 T . The concentration of PSA in shaking flask fermentation in creased from 1.09 g·L -1 to 2.24 g·L -1 after five rounds of mutagesis, while the concentration of PSA in 5L fermentor fermentation raised from 4.79 g·L -1 to 9.22 g·L -1 , increased by 92%. Therefore, the compound mutagenesis of magnetic field and Fe 2＋ showed obvious effect on Escherichia coli of PSA fermentation.
作者：
吴金勇 陈祥松 袁丽霞 朱薇薇 孙立洁 李俊 高兰兰 姚建铭
Wu Jinyong;Chen Xiangsong;Yuan Lixia;Zhu Weiwei;Sun Lijie;Li Jun;Gao Lanlan;Yao Jianming(University of Chinese Academy of Sciences,Beijing 100049,China;Hefei institutes of Physical Science,Chinese Academy of Sciences,Hefei 230031,China;Huainan New Energy Research Center,Huainan,232001,China)
机构地区：
中国科学院大学 中国科学院合肥物质科学研究院 淮南新能源研究中心
出处：
《Betway官方客服学报：自然科学版》 CAS 北大核心 2018年第6期80-85,共6页
基金国家自然科学基金(215062351008217) 淮南市重点研究和开发专项(2017A044) 中科院等离子体物理研究所科学基金(DSJJ-16-YY02)
关键词：
聚唾液酸 磁场 亚铁离子 诱变
polysialic acid magnetic field Fe 2＋ compound mutagensis
分类号：
Q939.9 [生物学—微生物学]
